| Data | In casa | Ospite | Risultato |
|---|---|---|---|
| 05/12/25 20.30 | Salford City | Leyton Orient | 4:0 |
| 06/12/25 16.00 | Exeter City | Wycombe Wanderers | 4:0 |
| 06/12/25 16.00 | Cheltenham Town | Buxton FC | 6:2 |
| 06/12/25 16.00 | Accrington Stanley | Mansfield Town | 3:5d.c.r |
| 06/12/25 16.00 | Stockport County | Cambridge United | 4:5d.c.r |
| 06/12/25 16.00 | Milton Keynes Dons FC | Oldham Athletic | 3:1 |
| 06/12/25 16.00 | Wigan Athletic | Barrow FC | 6:5d.c.r |
| 06/12/25 16.00 | Fleetwood Town | Luton Town | 6:4d.c.r |
| 06/12/25 16.00 | Swindon Town | Bolton Wanderers | 4:0 |
| 06/12/25 16.00 | Chelmsford City | Weston-Super-Mare | 0:2 |
| 06/12/25 16.00 | Peterborough United | FC Barnsley | 0:1 |
| 06/12/25 16.00 | Grimsby Town | Wealdstone FC | 4:0 |
| 06/12/25 16.00 | FC Port Vale | Bristol Rovers | 1:0 |
| 06/12/25 18.15 | Sutton United | Shrewsbury Town | 1:2d.t.s |
| 06/12/25 20.30 | FC Chesterfield | Doncaster Rovers | 1:2 |
| 07/12/25 13.30 | Slough Town FC | Macclesfield FC | 1:3d.t.s |
| 07/12/25 15.30 | Boreham Wood | Newport County | 3:0 |
| 07/12/25 16.30 | FC Gateshead | FC Walsall | 0:2 |
| 07/12/25 18.30 | FC Blackpool | Carlisle United | 4:1 |
| 08/12/25 20.30 | Brackley Town | Burton Albion | 1:3 |